Mechanics of composite vimentin-microtubule systems

The shape of cells is defined by the internal cytoskeleton, that consists of microtubules, actin filaments and intermediate filaments (IFs). This composite network of proteins enables cells to change their shape, divide, adapt to changing circumstances, and move around. Each type of filament that structures the cytoskeleton has distinct mechanical properties and dynamics, but only combined together, they maintain in a proper way internal order. The mechanical properties of individual actin filaments and microtubules as well as networks composed of those two proteins have been studied for over 50 years. However intermediate filaments, and especially their interactions with other cytoskeletal components, are not well described yet. In general, the major function of IF is to give physical strength to cells and tissues.
In our study we probe mechanical properties of composite vimentin (type of IFs) and microtubule networks using rheology. To complement rheological measurements, we visualize networks in steady state as well under deformation using a home built shear cell device placed on top of a confocal microscope.

More information: Izabela Piechocka

Collaborators: Dr. Harald Herrmann (DKFZ, Heidelberg)